Hotspot

rpl16

LSC · 85073-86400 · 1328 bp

Back to Khaya

Pi 0.0012
MarkerSeek score 65.60
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1328
variable_sites
4
pi
0.0012
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGTAAAGTAAAGCAAAGGACGA TCCGCAATTCTATAGGGTTGA 36.4 42.9 56.0 56.5 1401-1544 bp 1.0 44.2
rpl16_p2 AAGTAAAGCAAAGGACGAAG TCCGCAATTCTATAGGGTTGA 40.0 42.9 53.9 56.5 1397-1540 bp 1.0 44.2
rpl16_p3 TCCAAGATTTCTTTCCTTTGGT TCCGCAATTCTATAGGGTTGA 36.4 42.9 55.8 56.5 1339-1482 bp 1.0 44.2
rpl16_p4 TGATGTAAAGTAAAGCAAAGGACG TCCGCAATTCTATAGGGTTGA 37.5 42.9 57.8 56.5 1404-1547 bp 1.0 44.2
rpl16_p5 AAAGTAAAGCAAAGGACGAAG TCCGCAATTCTATAGGGTTGA 38.1 42.9 54.7 56.5 1398-1541 bp 1.0 44.2