Hotspot

clpP1

LSC · 73689-75800 · 2112 bp

Pi 0.00142

MarkerSeek score 59.10

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2112
variable_sites: 7
pi: 0.00142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TCGTGCATTTGCGTGAAATC	AGTCCATTATTACGCTTCCACA	45.0	40.9	58.0	57.5	2200-2208 bp	1.0	60.5
clpP1_p2	TCGTGCATTTGCGTGAAATC	ACGCTTCCACATCAAAGTAAAC	45.0	40.9	58.0	57.5	2189-2197 bp	1.0	60.5
clpP1_p3	TCGTGCATTTGCGTGAAATC	ACGCTTCCACATCAAAGTAA	45.0	40.0	58.0	55.3	2189-2197 bp	1.0	58.8
clpP1_p4	TCGTGCATTTGCGTGAAATC	TTACGCTTCCACATCAAAGT	45.0	40.0	58.0	55.3	2191-2199 bp	1.0	58.8
clpP1_p5	TCGTGCATTTGCGTGAAATC	CGCTTCCACATCAAAGTAAACT	45.0	40.9	58.0	57.3	2188-2196 bp	1.0	58.4