Hotspot

rpl32-trnL

SSC · 117859-118953 · 1095 bp

Back to Khaya

Pi 0.00311
MarkerSeek score 39.50
Primer available yes
Label rpl32-trnL

Feature Metadata

label_name
rpl32-trnL
feature_type
igs
region
SSC
length_bp
1095
variable_sites
8
pi
0.00311
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL_p1 CGAAATCCCTTTCTACCGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1174-1247 bp 1.0 80.3
rpl32-trnL_p2 CGAAATCCCTTTCTACCGGG GAGCGGGTACTAATATGCCG 55.0 55.0 58.1 58.0 1721-1794 bp 1.0 80.3
rpl32-trnL_p3 CGAAATCCCTTTCTACCGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1175-1248 bp 1.0 80.3
rpl32-trnL_p4 CGAAATCCCTTTCTACCGGG GAAGTAGCAAAGCCTTGGGT 55.0 50.0 58.1 58.1 1674-1747 bp 1.0 79.7
rpl32-trnL_p5 CGAAATCCCTTTCTACCGGG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 1184-1257 bp 1.0 79.3