Hotspot

psbE-petL

LSC · 68290-69562 · 1273 bp

Back to Khaya

Pi 0.00197
MarkerSeek score 60.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1273
variable_sites
6
pi
0.00197
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GACCAATAAATAGAGCCGAGGT 50.0 45.5 57.9 57.7 1448-1564 bp 1.0 61.4
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GACCAATAAATAGAGCCGAGGT 50.0 45.5 57.8 57.7 1371-1487 bp 1.0 60.6
psbE-petL_p3 ACGAATAACCAACCCGCAAT GACCAATAAATAGAGCCGAGGT 45.0 45.5 58.2 57.7 1457-1573 bp 1.0 60.4
psbE-petL_p4 TGCTGACGAATAACCAACCC GACCAATAAATAGAGCCGAGGT 50.0 45.5 58.2 57.7 1462-1578 bp 1.0 60.3
psbE-petL_p5 CGAATAACCAACCCGCAATG GACCAATAAATAGAGCCGAGGT 50.0 45.5 57.8 57.7 1456-1572 bp 1.0 60.3