Hotspot

petA-psbJ

LSC · 66444-67504 · 1061 bp

Back to Khaya

Pi 0.00415
MarkerSeek score 55.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1061
variable_sites
9
pi
0.00415
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCATCTGTTGTTTTGGCACA CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 903-1168 bp 1.0 82.4
petA-psbJ_p2 TTGGCATCTGTTGTTTTGGC CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.8 58.1 906-1171 bp 1.0 82.2
petA-psbJ_p3 GCATCTGTTGTTTTGGCACA TCCGGATTAGGTTCGTCCTT 45.0 50.0 57.8 58.1 904-1169 bp 1.0 81.7
petA-psbJ_p4 TTGGCATCTGTTGTTTTGGC TCCGGATTAGGTTCGTCCTT 45.0 50.0 57.8 58.1 907-1172 bp 1.0 81.6
petA-psbJ_p5 TGTCCAAGGCCTTTTGTTCT CCGGATTAGGTTCGTCCTTG 45.0 55.0 57.5 58.1 928-1193 bp 1.0 80.5