Hotspot

ycf4-cemA

LSC · 63638-64552 · 915 bp

Back to Khaya

Pi 0.00153
MarkerSeek score 63.60
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
915
variable_sites
3
pi
0.00153
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT AGATCCACCAGGGCAAAAAG 45.0 50.0 58.1 58.1 679-1033 bp 1.0 82.9
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT GAGATCCACCAGGGCAAAAA 45.0 50.0 58.1 58.1 680-1034 bp 1.0 82.9
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT GATCCACCAGGGCAAAAAGA 45.0 50.0 58.1 58.1 678-1032 bp 1.0 82.9
ycf4-cemA_p4 TTGGCCTATTTCTTGCGTGT CCACCAGGGCAAAAAGACTA 45.0 50.0 58.1 57.7 675-1029 bp 1.0 81.2
ycf4-cemA_p5 TTGGCCTATTTCTTGCGTGT GAGAGAGAGATCCACCAGGG 45.0 60.0 58.1 58.3 686-1040 bp 1.0 81.1