Hotspot

ndhC-trnV

LSC · 53568-54470 · 903 bp

Back to Khaya

Pi 0.00399
MarkerSeek score 54.30
Primer available yes
Label ndhC-trnV

Feature Metadata

label_name
ndhC-trnV
feature_type
igs
region
LSC
length_bp
903
variable_sites
9
pi
0.00399
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV_p1 TCTGGCCCTTTGTTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1052-1205 bp 1.0 78.1
ndhC-trnV_p2 TAATCGGGGCTAAAACTGCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1039-1192 bp 1.0 76.4
ndhC-trnV_p3 TCTGGCCCTTTGTTAATCGG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1051-1204 bp 1.0 75.4
ndhC-trnV_p4 TCTGGCCCTTTGTTAATCGG TGGGTCTTTGAAAGAGTCCG 50.0 50.0 57.9 57.5 1110-1263 bp 1.0 75.3
ndhC-trnV_p5 TCTGGCCCTTTGTTAATCGG GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1040-1193 bp 1.0 74.2