Hotspot

psbZ-trnG

LSC · 38402-38982 · 581 bp

Back to Khaya

Pi 0.00724
MarkerSeek score 69.50
Primer available yes
Label psbZ-trnG

Feature Metadata

label_name
psbZ-trnG
feature_type
igs
region
LSC
length_bp
581
variable_sites
10
pi
0.00724
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG_p1 CGGATGGTTGGTCGAGTAAC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.4 57.8 689-729 bp 1.0 80.8
psbZ-trnG_p2 TTTGCTTCTCCGGATGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.5 57.8 699-739 bp 1.0 80.0
psbZ-trnG_p3 ATTTGCTTCTCCGGATGGTT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.5 57.8 700-740 bp 1.0 79.6
psbZ-trnG_p4 GGATGGTTGGTCGAGTAACA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.2 57.8 688-728 bp 1.0 77.7
psbZ-trnG_p5 CGTTGTATTTGCTTCTCCGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.2 57.8 706-746 bp 1.0 77.3