Hotspot

trnK-rps16

LSC · 4450-5316 · 867 bp

Back to Khaya

Pi 0.00369
MarkerSeek score 54.20
Primer available yes
Label trnK-rps16

Feature Metadata

label_name
trnK-rps16
feature_type
igs
region
LSC
length_bp
867
variable_sites
8
pi
0.00369
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-rps16_p1 AGCCGTACTTAAAAGCCGAG AGGTAGGGGTTTTTACAGAACT 50.0 40.9 58.0 56.6 943-964 bp 1.0 55.5
trnK-rps16_p2 AAAGCCGAGTACTCTACCGT AGGTAGGGGTTTTTACAGAACT 50.0 40.9 58.2 56.6 932-953 bp 1.0 54.3
trnK-rps16_p3 AAGCCGAGTACTCTACCGTT AGGTAGGGGTTTTTACAGAACT 50.0 40.9 58.2 56.6 931-952 bp 1.0 54.3
trnK-rps16_p4 GCCGTACTTAAAAGCCGAGT AGGTAGGGGTTTTTACAGAACT 50.0 40.9 58.3 56.6 942-963 bp 1.0 53.3
trnK-rps16_p5 AGCCGTACTTAAAAGCCGAG AGGTAGGGGTTTTTACAGAACTT 50.0 39.1 58.0 57.3 943-964 bp 1.0 53.1