Hotspot

ycf1

Genome · 93460-99120 · 5661 bp

Back to Keteleeria

Pi 0.000624

MarkerSeek score 53.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5661
variable_sites: 10
pi: 0.000624
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAAAAGATTTGTTGAAAATGCC	ACGTTTCTACCGTCGATTCG	33.3	50.0	57.4	58.1	204 bp	1.0	43.7
ycf1_p2	CCAAAAGATTTGTTGAAAATGCCT	ACGTTTCTACCGTCGATTCG	33.3	50.0	57.4	58.1	203 bp	1.0	43.7
ycf1_p3	TCCAAAAGATTTGTTGAAAATGCC	TTTTATGGTACCTGTGGGCG	33.3	50.0	57.4	57.9	226 bp	1.0	43.5
ycf1_p4	CCAAAAGATTTGTTGAAAATGCCT	TTTTATGGTACCTGTGGGCG	33.3	50.0	57.4	57.9	225 bp	1.0	43.5
ycf1_p5	TGGTAACCTGATCTATCCAAAAGA	ACGTTTCTACCGTCGATTCG	37.5	50.0	57.1	58.1	219 bp	1.0	41.5