Hotspot

rpoC2

Genome · 38390-42058 · 3669 bp

Back to Keteleeria

Pi 0.000545

MarkerSeek score 48.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: Genome
length_bp: 3669
variable_sites: 6
pi: 0.000545
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGTGACACAAGTATTCCATTGGT	CGAAAACAGTCGGATGGTGA	39.1	50.0	58.0	58.2	350 bp	1.0	54.7
rpoC2_p2	AGTGACACAAGTATTCCATTGGT	TCGAAAACAGTCGGATGGTG	39.1	50.0	58.0	58.2	351 bp	1.0	54.7
rpoC2_p3	AGTGACACAAGTATTCCATTGGT	ACGATTCGAAAACAGTCGGA	39.1	45.0	58.0	57.6	356 bp	1.0	52.9
rpoC2_p4	AGTGACACAAGTATTCCATTGGT	TTCGAAAACAGTCGGATGGT	39.1	45.0	58.0	57.5	352 bp	1.0	52.0
rpoC2_p5	CCATTGGTTCAAACTTGTCTAGG	CGAAAACAGTCGGATGGTGA	43.5	50.0	57.6	58.2	335 bp	1.0	51.8