Hotspot

petD

Genome · 55091-56295 · 1205 bp

Back to Keteleeria

Pi 0.00083

MarkerSeek score 50.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: Genome
length_bp: 1205
variable_sites: 3
pi: 0.00083
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGTCCTCGGATTTCTCCTT	TCTCTGACAATATGGGTAGGGT	50.0	45.5	58.0	57.7	1392 bp	1.0	61.5
petD_p2	ATGGTCCTCGGATTTCTCCT	TCTCTGACAATATGGGTAGGGT	50.0	45.5	57.8	57.7	1393 bp	1.0	60.5
petD_p3	TGGTCCTCGGATTTCTCCTT	CTCTGACAATATGGGTAGGGT	50.0	47.6	58.0	56.2	1391 bp	1.0	57.5
petD_p4	ATGGTCCTCGGATTTCTCCT	CTCTGACAATATGGGTAGGGT	50.0	47.6	57.8	56.2	1392 bp	1.0	56.4
petD_p5	TGGTCCTCGGATTTCTCCTT	TCTCTGACAATATGGGTAGGG	50.0	47.6	58.0	55.9	1392 bp	1.0	55.2