Hotspot

trnV-GAC-rps12

Genome · 105899-107431 · 1533 bp

Back to Keteleeria

Pi 0.000664
MarkerSeek score 48.60
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
Genome
length_bp
1533
variable_sites
3
pi
0.000664
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1602-1629 bp 1.0 78.1
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1606-1633 bp 1.0 78.1
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1601-1628 bp 1.0 78.1
trnV-GAC-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1604-1631 bp 1.0 77.1
trnV-GAC-rps12_p5 GGCTCGAACTGATGACTTCC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.4 57.7 1612-1639 bp 1.0 76.6