Hotspot

rpl32-trnV-GAC

Genome · 104848-105826 · 979 bp

Back to Keteleeria

Pi 0.00157

MarkerSeek score 47.60

Primer available yes

Label rpl32-trnV-GAC

Feature Metadata

label_name: rpl32-trnV-GAC
feature_type: igs
region: Genome
length_bp: 979
variable_sites: 4
pi: 0.00157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnV-GAC_p1	GTACGCTTTTTCGGAACTGC	AGTTCTCCTTCCGAGGGTAG	50.0	55.0	58.1	57.9	1096-1101 bp	1.0	78.8
rpl32-trnV-GAC_p2	GTACGCTTTTTCGGAACTGC	CAGTTCTCCTTCCGAGGGTA	50.0	55.0	58.1	58.2	1097-1102 bp	1.0	78.7
rpl32-trnV-GAC_p3	GTACGCTTTTTCGGAACTGC	TTGACGTGGTGGAAGTCATC	50.0	50.0	58.1	57.8	1043-1048 bp	1.0	78.6
rpl32-trnV-GAC_p4	GTACGCTTTTTCGGAACTGC	CGTGGTGGAAGTCATCAGTT	50.0	50.0	58.1	57.8	1039-1044 bp	1.0	78.6
rpl32-trnV-GAC_p5	GTACGCTTTTTCGGAACTGC	CTTGACGTGGTGGAAGTCAT	50.0	50.0	58.1	57.8	1044-1049 bp	1.0	78.6