Hotspot

psbD-trnT-GGU

Genome · 80400-81592 · 1193 bp

Back to Keteleeria

Pi 0.000838

MarkerSeek score 50.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1193
variable_sites: 3
pi: 0.000838
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CCCACAAAAACGAAACGGTC	TGATGTGGGGCCCTTTTAAC	50.0	50.0	58.2	58.1	1369 bp	1.0	78.3
psbD-trnT-GGU_p2	CCCACAAAAACGAAACGGTC	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.2	57.9	1360 bp	1.0	78.0
psbD-trnT-GGU_p3	AACCCACAAAAACGAAACGG	TGATGTGGGGCCCTTTTAAC	45.0	50.0	57.7	58.1	1371 bp	1.0	77.4
psbD-trnT-GGU_p4	AACCCACAAAAACGAAACGG	GCCCTTTTAACTCAGCGGTA	45.0	50.0	57.7	57.9	1362 bp	1.0	77.1
psbD-trnT-GGU_p5	CCCACAAAAACGAAACGGTC	ATGTGGGGCCCTTTTAACTC	50.0	50.0	58.2	57.8	1367 bp	1.0	77.0