Hotspot

trnM-CAU-trnG-GCC

Genome · 76709-76866 · 158 bp

Back to Keteleeria

Pi 0
MarkerSeek score 26.90
Primer available yes
Label trnM-CAU-trnG-GCC

Feature Metadata

label_name
trnM-CAU-trnG-GCC
feature_type
igs
region
Genome
length_bp
158
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-trnG-GCC_p1 CTTGAGGTCACGGGTTCAAA ATGCGGGTATGGTTGAATGG 50.0 50.0 58.0 58.3 265-271 bp 1.0 77.2
trnM-CAU-trnG-GCC_p2 TGCGGAGTAGAGTAGTCTGG ATGCGGGTATGGTTGAATGG 55.0 50.0 58.0 58.3 305-311 bp 1.0 77.2
trnM-CAU-trnG-GCC_p3 GCGGAGTAGAGTAGTCTGGT ATGCGGGTATGGTTGAATGG 55.0 50.0 58.0 58.3 304-310 bp 1.0 77.2
trnM-CAU-trnG-GCC_p4 GGTGCGGAGTAGAGTAGTCT ATGCGGGTATGGTTGAATGG 55.0 50.0 58.0 58.3 307-313 bp 1.0 77.2
trnM-CAU-trnG-GCC_p5 CTTGAGGTCACGGGTTCAAA GCGGGTATGGTTGAATGGTA 50.0 50.0 58.0 57.7 263-269 bp 1.0 77.0