Hotspot

ycf3-psaA

Genome · 70911-71565 · 655 bp

Back to Keteleeria

Pi 0.00204

MarkerSeek score 54.80

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: Genome
length_bp: 655
variable_sites: 4
pi: 0.00204
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	CTCCGGGCAATTACATCGAA	CCAGGTTTGGCCCATTTTTC	50.0	50.0	58.1	57.8	802-806 bp	1.0	78.9
ycf3-psaA_p2	TCCGGGCAATTACATCGAAG	CCAGGTTTGGCCCATTTTTC	50.0	50.0	58.1	57.8	801-805 bp	1.0	78.9
ycf3-psaA_p3	CAGGACGCTTTGGAGAATGA	CCAGGTTTGGCCCATTTTTC	50.0	50.0	57.9	57.8	760-764 bp	1.0	78.6
ycf3-psaA_p4	GCTGCTGAGTATTGGGAACA	CCAGGTTTGGCCCATTTTTC	50.0	50.0	57.9	57.8	836-840 bp	1.0	78.4
ycf3-psaA_p5	ACAGGACGCTTTGGAGAATG	CCAGGTTTGGCCCATTTTTC	50.0	50.0	58.2	57.8	761-765 bp	1.0	77.9