Hotspot

psbI-psbB

Genome · 50610-51066 · 457 bp

Back to Keteleeria

Pi 0

MarkerSeek score 46.90

Primer available yes

Label psbI-psbB

Feature Metadata

label_name: psbI-psbB
feature_type: igs
region: Genome
length_bp: 457
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbB_p1	CGTCCTGGATCGTTCGATAG	CAATTAACCGGCCAGGATCA	55.0	50.0	57.7	57.9	565-619 bp	1.0	81.0
psbI-psbB_p2	ACGTCCTGGATCGTTCGATA	CAATTAACCGGCCAGGATCA	50.0	50.0	58.3	57.9	566-620 bp	1.0	81.0
psbI-psbB_p3	TACGTCCTGGATCGTTCGAT	CAATTAACCGGCCAGGATCA	50.0	50.0	58.3	57.9	567-621 bp	1.0	81.0
psbI-psbB_p4	CGTCCTGGATCGTTCGATAG	TATGAACGCGATACCAAGGC	55.0	50.0	57.7	58.1	532-586 bp	1.0	80.3
psbI-psbB_p5	ACGTCCTGGATCGTTCGATA	TATGAACGCGATACCAAGGC	50.0	50.0	58.3	58.1	533-587 bp	1.0	80.3