Hotspot

trnS-GCU-ycf12

Genome · 8118-8629 · 512 bp

Back to Keteleeria

Pi 0.0207

MarkerSeek score 67.50

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: Genome
length_bp: 512
variable_sites: 31
pi: 0.0207
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	TAGTGACCGAACGGAGAGAG	TTACCTTTGCGAACTGCTGA	55.0	45.0	58.3	57.8	706-801 bp	1.0	79.3
trnS-GCU-ycf12_p2	CGGAGAGAGAGGGATTCGAA	TTACCTTTGCGAACTGCTGA	55.0	45.0	58.3	57.8	695-790 bp	1.0	79.0
trnS-GCU-ycf12_p3	ATAGTGACCGAACGGAGAGA	TTACCTTTGCGAACTGCTGA	50.0	45.0	57.6	57.8	707-802 bp	1.0	78.1
trnS-GCU-ycf12_p4	TAGTGACCGAACGGAGAGAG	ACCTTTGCGAACTGCTGATA	55.0	45.0	58.3	57.5	704-799 bp	1.0	77.5
trnS-GCU-ycf12_p5	TAGTGACCGAACGGAGAGAG	TACCTTTGCGAACTGCTGAT	55.0	45.0	58.3	57.5	705-800 bp	1.0	77.5