Hotspot

ycf1

SSC · 123893-129223 · 5331 bp

Back to Kernera

Pi 0.00131

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5331
variable_sites: 7
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTCATTCATTCCCATCCA	GGGTATAGGGGAAATTCGGC	40.0	55.0	54.0	57.8	219 bp	1.0	46.9
ycf1_p2	TCATTCATTCCCATCCAATCA	GGGTATAGGGGAAATTCGGC	38.1	55.0	54.9	57.8	216 bp	1.0	46.6
ycf1_p3	CTTCATTCATTCCCATCCAATCA	GGGTATAGGGGAAATTCGGC	39.1	55.0	56.9	57.8	218 bp	1.0	46.2
ycf1_p4	TCTTCATTCATTCCCATCCAA	GGGTATAGGGGAAATTCGGC	38.1	55.0	54.9	57.8	219 bp	1.0	46.0
ycf1_p5	TTCTTCATTCATTCCCATCCA	GGGTATAGGGGAAATTCGGC	38.1	55.0	54.9	57.8	220 bp	1.0	46.0