Hotspot

rpoB

LSC · 23297-26515 · 3219 bp

Back to Kernera

Pi 0.000621

MarkerSeek score 55.60

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3219
variable_sites: 2
pi: 0.000621
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TCCATGTCTTCCAGCTACTT	GTGGGTTGAAACGGGTGATA	45.0	50.0	55.9	57.8	286 bp	1.0	61.6
rpoB_p2	TTCCATGTCTTCCAGCTACT	GTGGGTTGAAACGGGTGATA	45.0	50.0	55.9	57.8	287 bp	1.0	61.6
rpoB_p3	TCCATGTCTTCCAGCTACTT	GAAGGCCGTTTACTCCGAAA	45.0	50.0	55.9	58.2	339 bp	1.0	61.4
rpoB_p4	TTCCATGTCTTCCAGCTACT	GAAGGCCGTTTACTCCGAAA	45.0	50.0	55.9	58.2	340 bp	1.0	61.4
rpoB_p5	TCCATGTCTTCCAGCTACTT	TTAGGGTCGTGGGTTGAAAC	45.0	50.0	55.9	57.7	294 bp	1.0	61.0