Hotspot

clpP

LSC · 69594-71673 · 2080 bp

Back to Kernera

Pi 0.000963

MarkerSeek score 58.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2080
variable_sites: 2
pi: 0.000963
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTTGCATGAAATCGCTCCT	CCCCAGTCTTACGTTTCCAC	42.9	55.0	57.3	58.2	2167-2171 bp	1.0	65.0
clpP_p2	ATTTGCATGAAATCGCTCCT	CCCCAGTCTTACGTTTCCAC	40.0	55.0	56.1	58.2	2166-2170 bp	1.0	63.3
clpP_p3	AGCAATTGTGGATTTGCATGA	CCCCAGTCTTACGTTTCCAC	38.1	55.0	57.0	58.2	2177-2181 bp	1.0	62.9
clpP_p4	GATTTGCATGAAATCGCTCCT	CCCAGTCTTACGTTTCCACA	42.9	50.0	57.3	57.5	2166-2170 bp	1.0	62.3
clpP_p5	GATTTGCATGAAATCGCTCCT	AACCCCAGTCTTACGTTTCC	42.9	50.0	57.3	57.4	2169-2173 bp	1.0	62.1