Hotspot

atpF

LSC · 11694-12972 · 1279 bp

Back to Kernera

Pi 0

MarkerSeek score 30.90

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1279
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTTTAATGGTTACC	AGTTTAGCTAGAAGAGGAGA	50.0	40.0	56.9	52.1	1398 bp	1.0	40.0
atpF_p2	CGTCGGCTTTAATGGTTACC	AGTTTAGCTAGAAGAGGAGAT	50.0	38.1	56.9	52.8	1398 bp	1.0	40.0
atpF_p3	TCGTCGGCTTTAATGGTTACC	AGTTTAGCTAGAAGAGGAGA	47.6	40.0	58.4	52.1	1399 bp	1.0	40.0
atpF_p4	CGTCGGCTTTAATGGTTACC	AGTTTAGCTAGAAGAGGAGATT	50.0	36.4	56.9	53.7	1398 bp	1.0	40.0
atpF_p5	TCGTCGGCTTTAATGGTTACC	AGTTTAGCTAGAAGAGGAGAT	47.6	38.1	58.4	52.8	1399 bp	1.0	40.0