Hotspot

ndhF-rpl32

SSC · 112663-113412 · 750 bp

Back to Kernera

Pi 0.00134
MarkerSeek score 59.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
750
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA AACGCTTTCAACGATGTCCA 47.6 45.0 57.6 58.4 897-1063 bp 1.0 65.4
ndhF-rpl32_p2 CTGGAAGTGGAATGAAAGGGA ACGCTTTCAACGATGTCCAA 47.6 45.0 57.6 58.4 896-1062 bp 1.0 65.4
ndhF-rpl32_p3 TGGAAGTGGAATGAAAGGGA AACGCTTTCAACGATGTCCA 45.0 45.0 56.3 58.4 896-1062 bp 1.0 63.6
ndhF-rpl32_p4 TGGAAGTGGAATGAAAGGGA ACGCTTTCAACGATGTCCAA 45.0 45.0 56.3 58.4 895-1061 bp 1.0 63.6
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGGA AAACGCTTTCAACGATGTCC 47.6 45.0 57.6 57.3 898-1064 bp 1.0 63.3