Hotspot

ycf3-trnS-UCC

LSC · 43844-44930 · 1087 bp

Back to Kernera

Pi 0.00092

MarkerSeek score 59.30

Primer available yes

Label ycf3-trnS-UCC

Feature Metadata

label_name: ycf3-trnS-UCC
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 1
pi: 0.00092
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-UCC_p1	CCTTCAGATTGAGCCGACAT	TGTTACGCCTTCAACCACTC	50.0	50.0	57.7	58.1	1139 bp	1.0	76.5
ycf3-trnS-UCC_p2	CCTTCAGATTGAGCCGACAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.7	58.2	1195 bp	1.0	76.3
ycf3-trnS-UCC_p3	TTCCTTCAGATTGAGCCGAC	TGTTACGCCTTCAACCACTC	50.0	50.0	57.6	58.1	1141 bp	1.0	76.0
ycf3-trnS-UCC_p4	GGGGTCAATTTCTAGTCGCA	TGTTACGCCTTCAACCACTC	50.0	50.0	57.6	58.1	1197 bp	1.0	75.9
ycf3-trnS-UCC_p5	TTCCTTCAGATTGAGCCGAC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.6	58.2	1197 bp	1.0	75.8