Hotspot

atpF-atpH

LSC · 12973-13451 · 479 bp

Back to Kernera

Pi 0.00835

MarkerSeek score 61.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 4
pi: 0.00835
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	597-598 bp	1.0	76.2
atpF-atpH_p2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.5	57.6	570-571 bp	1.0	72.9
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	50.0	45.0	57.9	57.1	601-602 bp	1.0	72.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	50.0	55.0	57.9	59.0	596-597 bp	1.0	71.8
atpF-atpH_p5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	55.0	50.0	58.8	57.6	572-573 bp	1.0	71.2