Hotspot

matK-trnK-AAA

LSC · 3696-4368 · 673 bp

Back to Kernera

Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label matK-trnK-AAA

Feature Metadata

label_name: matK-trnK-AAA
feature_type: igs
region: LSC
length_bp: 673
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-AAA_p1	CCGAGCTCCATCGAACTCTA	AACGGTAGAGTACTCGGCTT	55.0	50.0	58.7	58.2	805 bp	1.0	73.2
matK-trnK-AAA_p2	CCGAGCTCCATCGAACTCTA	ACGGTAGAGTACTCGGCTTT	55.0	50.0	58.7	58.2	804 bp	1.0	73.2
matK-trnK-AAA_p3	AAAACTCTGTTGCCGAGCTC	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.8	58.2	817 bp	1.0	72.6
matK-trnK-AAA_p4	AAAACTCTGTTGCCGAGCTC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.8	58.2	816 bp	1.0	72.6
matK-trnK-AAA_p5	CCGAGCTCCATCGAACTCTA	GGGTTGCTAACTCAACGGTA	55.0	50.0	58.7	57.5	818 bp	1.0	71.0