Hotspot

trnH-CAC-psbA

LSC · 77-409 · 333 bp

Back to Kernera

Pi 0.00316

MarkerSeek score 59.80

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 333
variable_sites: 1
pi: 0.00316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	GACCTAGCTTCTGTTGAGGC	50.0	55.0	57.9	58.0	388-400 bp	1.0	82.4
trnH-CAC-psbA_p2	ACTGCCTTAATCCACTTGGC	GACCTAGCTTCTGTTGAGGC	50.0	55.0	58.2	58.0	389-401 bp	1.0	82.2
trnH-CAC-psbA_p3	AATCCACTTGGCTACATCCG	GACCTAGCTTCTGTTGAGGC	50.0	55.0	57.7	58.0	381-393 bp	1.0	80.9
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	446-458 bp	1.0	79.4
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	447-459 bp	1.0	79.2