Hotspot

ndhH

SSC · 113273-114454 · 1182 bp

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Pi 0.00302

MarkerSeek score 48.90

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 8
pi: 0.00302
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGAGGGTCAAAATGGGTAGA	TTTGATGAGCCTCGCTTTGA	47.6	45.0	58.1	57.8	1364 bp	1.0	70.4
ndhH_p2	AAAGGAGGGTCAAAATGGGT	TTTGATGAGCCTCGCTTTGA	45.0	45.0	56.9	57.8	1366 bp	1.0	70.2
ndhH_p3	TCCCAAAAGGAGGGTCAAAA	TTTGATGAGCCTCGCTTTGA	45.0	45.0	56.8	57.8	1371 bp	1.0	69.7
ndhH_p4	AGGAGGGTCAAAATGGGTAGA	TTGATGAGCCTCGCTTTGAG	47.6	50.0	58.1	58.3	1363 bp	1.0	69.7
ndhH_p5	AAAGGAGGGTCAAAATGGGT	TTGATGAGCCTCGCTTTGAG	45.0	50.0	56.9	58.3	1365 bp	1.0	69.6