Hotspot

ccsA

SSC · 105724-106692 · 969 bp

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Pi 0.00158

MarkerSeek score 48.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 5
pi: 0.00158
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TTTGAGAAGGCGTTCAAGGG	50.0	50.0	58.0	58.4	1154-1160 bp	1.0	77.2
ccsA_p2	AAGCATCTCGGTTCGAATCC	TTTGAGAAGGCGTTCAAGGG	50.0	50.0	58.1	58.4	1151-1157 bp	1.0	76.7
ccsA_p3	GTGGCGGCATTCTTGAAAAA	TTTGAGAAGGCGTTCAAGGG	45.0	50.0	57.9	58.4	1129-1135 bp	1.0	76.0
ccsA_p4	TTAGGAAGCAGTGCTCAAGC	TTTGAGAAGGCGTTCAAGGG	50.0	50.0	58.2	58.4	1167-1173 bp	1.0	75.7
ccsA_p5	CTCAAGCATCTCGGTTCGAA	GCGTTCAAGGGGTTCTCAAA	50.0	50.0	58.0	58.7	1145-1151 bp	1.0	74.8