Hotspot

trnF-GAA-ndhJ

LSC · 47532-48112 · 581 bp

Back to Kengyilia

Pi 0.00443

MarkerSeek score 49.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 5
pi: 0.00443
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	50.0	50.0	58.1	57.9	716-728 bp	1.0	79.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	691-703 bp	1.0	79.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	681-693 bp	1.0	78.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	50.0	45.0	58.1	57.5	728-740 bp	1.0	76.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	50.0	50.0	58.1	58.5	719-731 bp	1.0	76.1