Hotspot

petN-trnC-GCA

LSC · 17622-18110 · 489 bp

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Pi 0.00226

MarkerSeek score 48.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 2
pi: 0.00226
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	589-601 bp	1.0	72.1
petN-trnC-GCA_p2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	588-600 bp	1.0	72.1
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	530-542 bp	1.0	72.0
petN-trnC-GCA_p4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	591-603 bp	1.0	71.6
petN-trnC-GCA_p5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	590-602 bp	1.0	71.6