Hotspot

psbZ-trnG-UCC

LSC · 11817-12093 · 277 bp

Back to Kengyilia

Pi 0.00361

MarkerSeek score 34.10

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 4
pi: 0.00361
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	TACCATTGAACTACGCTCGC	50.0	50.0	57.8	58.1	407-412 bp	1.0	78.5
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	TACCATTGAACTACGCTCGC	55.0	50.0	58.3	58.1	405-410 bp	1.0	78.1
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	50.0	50.0	57.8	57.6	443-448 bp	1.0	75.6
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	55.0	50.0	58.3	57.6	441-446 bp	1.0	75.2
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	GATAAATTAAGCGGGCGAGC	50.0	50.0	57.8	57.4	471-476 bp	1.0	74.3