primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	ACCCAATATCTTGCTAGAACAAG	AAATGCAAGCACGATTTGGG	23	20	39.1	45.0	56.3	57.9	1.0	0.0	4.758548	92	1153	1473-1473	0.500000	22.1
psbA_p2	psbA	2	ACCCAATATCTTGCTAGAACAAG	GCGCTAGAACTTTAGCTCGT	23	20	39.1	50.0	56.3	58.1	1.0	0.0	4.764694	92	1153	1892-1892	0.500000	22.1
psbA_p3	psbA	3	ACCCAATATCTTGCTAGAACAAG	TGCGCTAGAACTTTAGCTCG	23	20	39.1	50.0	56.3	58.1	1.0	9.7	4.766006	92	1153	1893-1893	0.500000	22.1
psbA_p4	psbA	4	ACCCAATATCTTGCTAGAACA	AAATGCAAGCACGATTTGGG	21	20	38.1	45.0	54.3	57.9	1.0	0.0	4.765156	92	1153	1473-1473	0.500000	22.0
psbA_p5	psbA	5	TGCTAGAACAAGATATTGGGT	AAATGCAAGCACGATTTGGG	21	20	38.1	45.0	54.3	57.9	4.9	0.0	4.765156	92	1153	1462-1462	0.500000	22.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	21	20	47.6	45.0	58.3	58.0	0.0	0.0	1.340971	3223	3911	876-876	1.000000	69.7
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACTCCTGAAAGAGAAGTGGG	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	56.5	58.0	0.0	0.0	1.570959	3223	3911	876-876	1.000000	67.9
matK-trnK-UUU_p3	matK-trnK-UUU	3	CTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	20	20	50.0	45.0	56.5	58.0	0.0	0.0	1.570959	3223	3911	875-875	1.000000	67.9
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCCTGAAAGAGAAGTGGGTAGA	AATGTTTTGACAGAAGCGCG	22	20	45.5	45.0	57.9	58.0	0.0	0.0	2.164474	3223	3911	874-874	1.000000	63.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACTCCTGAAAGAGAAGTGGGTA	AATGTTTTGACAGAAGCGCG	22	20	45.5	45.0	58.1	58.0	0.0	0.0	2.178422	3223	3911	876-876	1.000000	63.0
rps16_p1	rps16	1	TGGCACGATATAAATCCAAATAGG	TGCTCCATAGATAGCAAGTT	24	20	37.5	40.0	56.9	53.8	0.0	0.0	9.241165	4509	5577	1288-1300	1.000000	40.4
rps16_p2	rps16	2	TGGCACGATATAAATCCAAATAGG	TCCATATTGCTCCATAGATAGCA	24	23	37.5	39.1	56.9	56.7	0.0	0.0	9.345307	4509	5577	1295-1307	1.000000	40.4
rps16_p3	rps16	3	TGGCACGATATAAATCCAAATAGG	TGCTCCATAGATAGCAAGTTT	24	21	37.5	38.1	56.9	54.7	0.0	0.0	9.356523	4509	5577	1288-1300	1.000000	40.4
rps16_p4	rps16	4	TGGCACGATATAAATCCAAA	TGCTCCATAGATAGCAAGTT	20	20	35.0	40.0	52.6	53.8	0.0	0.0	9.545978	4509	5577	1288-1300	1.000000	40.4
rps16_p5	rps16	5	TTGGCACGATATAAATCCAA	TGCTCCATAGATAGCAAGTT	20	20	35.0	40.0	52.6	53.8	0.0	0.0	9.545978	4509	5577	1289-1301	1.000000	40.4
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	TACCATTGAACTACGCTCGC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.287750	11817	12093	407-412	1.000000	78.5
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	GCTTCTCCTGATGGTTGGTC	TACCATTGAACTACGCTCGC	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.338732	11817	12093	405-410	1.000000	78.1
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.636976	11817	12093	443-448	1.000000	75.6
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	20	20	55.0	50.0	58.3	57.6	0.0	0.0	0.687958	11817	12093	441-446	1.000000	75.2
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TTGCTTCTCCTGATGGTTGG	GATAAATTAAGCGGGCGAGC	20	20	50.0	50.0	57.8	57.4	0.0	0.0	0.801286	11817	12093	471-476	1.000000	74.3
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	AGTGGTAGAGTAATCTCGTGC	GCTGCCTCCTTGAAAGAGAG	21	20	47.6	55.0	56.9	58.3	0.0	0.0	2.322963	14785	15248	567-568	1.000000	62.3
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	GTGGTAGAGTAATCTCGTGCT	GCTGCCTCCTTGAAAGAGAG	21	20	47.6	55.0	56.9	58.3	0.0	0.0	2.322963	14785	15248	566-567	1.000000	62.3
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	GTGGTAGAGTAATCTCGTGCT	TGTCCTGAACCACTAGACGA	21	20	47.6	50.0	56.9	57.7	0.0	0.0	2.341113	14785	15248	545-546	1.000000	62.2
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	AGTGGTAGAGTAATCTCGTGC	TGTCCTGAACCACTAGACGA	21	20	47.6	50.0	56.9	57.7	0.0	0.0	2.341113	14785	15248	546-547	1.000000	62.1
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	GAGTAATCTCGTGCTAAGACGT	GCTGCCTCCTTGAAAGAGAG	22	20	45.5	55.0	57.7	58.3	0.0	0.0	2.539227	14785	15248	560-561	1.000000	60.5
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	17622	18110	589-601	1.000000	72.1
petN-trnC-GCA_p2	petN-trnC-GCA	2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.1	58.1	0.0	0.0	1.172113	17622	18110	588-600	1.000000	72.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	17622	18110	530-542	1.000000	72.0
petN-trnC-GCA_p4	petN-trnC-GCA	4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	17622	18110	591-603	1.000000	71.6
petN-trnC-GCA_p5	petN-trnC-GCA	5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	20	20	55.0	55.0	58.1	59.1	0.0	4.3	1.228376	17622	18110	590-602	1.000000	71.6
trnR-UCU-trnfM-CAU_p1	trnR-UCU-trnfM-CAU	1	AGAAGACCTCTGTCCTATCCA	GGCGTATCCAACCCTCTTTT	21	20	47.6	50.0	56.9	57.9	0.0	0.0	2.175882	35823	35985	302-309	1.000000	65.0
trnR-UCU-trnfM-CAU_p2	trnR-UCU-trnfM-CAU	2	AGGTTTAGAAGACCTCTGTCCT	GGCGTATCCAACCCTCTTTT	22	20	45.5	50.0	57.9	57.9	0.0	0.0	2.259210	35823	35985	308-315	1.000000	64.3
trnR-UCU-trnfM-CAU_p3	trnR-UCU-trnfM-CAU	3	AGAAGACCTCTGTCCTATCCA	AAGGCTCATAACCTTGGGGT	21	20	47.6	50.0	56.9	58.6	0.0	0.0	2.668690	35823	35985	228-235	1.000000	61.8
trnR-UCU-trnfM-CAU_p4	trnR-UCU-trnfM-CAU	4	AGAAGACCTCTGTCCTATCCAT	GGCGTATCCAACCCTCTTTT	22	20	45.5	50.0	57.4	57.9	0.0	0.0	2.675493	35823	35985	302-309	1.000000	61.0
trnR-UCU-trnfM-CAU_p5	trnR-UCU-trnfM-CAU	5	GGTTTAGAAGACCTCTGTCCT	GGCGTATCCAACCCTCTTTT	21	20	47.6	50.0	56.4	57.9	0.0	0.0	2.744897	35823	35985	307-314	1.000000	60.4
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175907	47532	48112	716-728	1.000000	79.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	47532	48112	691-703	1.000000	79.1
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	47532	48112	681-693	1.000000	78.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	20	20	50.0	45.0	58.1	57.5	0.0	0.0	0.531462	47532	48112	728-740	1.000000	76.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.557071	47532	48112	719-731	1.000000	76.1
rbcL-psaI_p1	rbcL-psaI	1	CCGAAATCTTTGGGGACGAT	AACTCGCTATACCTGGCTCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.113115	55873	57515	2159-2196	1.000000	79.6
rbcL-psaI_p2	rbcL-psaI	2	TCTTCACATTCACCGTGCAA	AACTCGCTATACCTGGCTCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.114312	55873	57515	2461-2498	1.000000	79.6
rbcL-psaI_p3	rbcL-psaI	3	TTCTTCACATTCACCGTGCA	AACTCGCTATACCTGGCTCT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.114312	55873	57515	2462-2499	1.000000	79.6
rbcL-psaI_p4	rbcL-psaI	4	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	20	20	50.0	45.0	58.0	57.9	0.0	26.9	0.115800	55873	57515	1973-2010	1.000000	79.5
rbcL-psaI_p5	rbcL-psaI	5	CTTAACTGGGGGATTCACCG	AACTCGCTATACCTGGCTCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.113115	55873	57515	2525-2561	0.875000	74.5
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	58.0	57.1	0.0	0.0	0.895979	60866	61686	1015-1016	1.000000	73.1
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	57.9	57.1	0.0	0.0	0.909471	60866	61686	998-999	1.000000	73.0
petA-psbJ_p3	petA-psbJ	3	TGTTCTTCTTCGCATCCGTT	GCTGGTATTGCTGTGATTGG	20	20	45.0	50.0	57.8	57.1	0.0	0.0	1.021410	60866	61686	991-992	1.000000	72.2
petA-psbJ_p4	petA-psbJ	4	TTGTTCTTCTTCGCATCCGT	GCTGGTATTGCTGTGATTGG	20	20	45.0	50.0	57.8	57.1	0.0	0.0	1.021410	60866	61686	992-993	1.000000	72.2
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	GCTGGTATTGCTGTGATTGG	20	20	50.0	50.0	58.2	57.1	13.8	0.0	1.036375	60866	61686	1012-1013	1.000000	72.0
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	20	20	45.0	50.0	58.0	58.3	0.0	7.6	0.278273	62456	63624	1363-1373	1.000000	78.9
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	57.9	58.3	6.4	7.6	0.312268	62456	63624	1352-1362	1.000000	78.7
psbE-petL_p3	psbE-petL	3	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	62456	63624	1317-1327	1.000000	77.3
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	62456	63624	1315-1325	1.000000	77.2
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	20	20	50.0	50.0	58.2	58.3	0.0	7.6	0.498226	62456	63624	1354-1364	1.000000	77.2
rps8-rpl14_p1	rps8-rpl14	1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	20	20	50.0	50.0	57.9	58.1	3.8	0.0	0.173886	77176	77320	226-236	1.000000	82.4
rps8-rpl14_p2	rps8-rpl14	2	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.292267	77176	77320	223-233	1.000000	81.5
rps8-rpl14_p3	rps8-rpl14	3	TCAGCAATAGTGTCCTTGCC	GGCAATCCAAAAGGAACTCG	20	20	50.0	50.0	57.9	57.4	3.8	0.0	0.750628	77176	77320	257-267	1.000000	77.4
rps8-rpl14_p4	rps8-rpl14	4	GCAATAGTGTCCTTGCCCAT	GGCAATCCAAAAGGAACTCG	20	20	50.0	50.0	58.2	57.4	0.0	0.0	0.869009	77176	77320	254-264	1.000000	76.5
rps8-rpl14_p5	rps8-rpl14	5	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	20	20	50.0	50.0	57.9	58.8	3.8	30.2	0.887114	77176	77320	246-256	1.000000	76.4
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	20	24	55.0	29.2	58.1	55.3	0.0	0.0	6.833076	103770	104679	1019-1026	1.000000	41.3
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	103770	104679	992-999	1.000000	41.3
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	103770	104679	993-1000	1.000000	41.3
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	103770	104679	994-1001	1.000000	41.3
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	103770	104679	995-1002	1.000000	41.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050149	104872	105572	840-847	1.000000	80.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105116	104872	105572	843-850	1.000000	80.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	104872	105572	812-819	1.000000	80.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	104872	105572	811-818	1.000000	80.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.170718	104872	105572	813-820	1.000000	79.7
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	TTTGAGAAGGCGTTCAAGGG	20	20	50.0	50.0	58.0	58.4	0.0	20.4	0.399105	105724	106692	1154-1160	1.000000	77.2
ccsA_p2	ccsA	2	AAGCATCTCGGTTCGAATCC	TTTGAGAAGGCGTTCAAGGG	20	20	50.0	50.0	58.1	58.4	0.0	20.4	0.454071	105724	106692	1151-1157	1.000000	76.7
ccsA_p3	ccsA	3	GTGGCGGCATTCTTGAAAAA	TTTGAGAAGGCGTTCAAGGG	20	20	45.0	50.0	57.9	58.4	17.2	20.4	0.542832	105724	106692	1129-1135	1.000000	76.0
ccsA_p4	ccsA	4	TTAGGAAGCAGTGCTCAAGC	TTTGAGAAGGCGTTCAAGGG	20	20	50.0	50.0	58.2	58.4	20.0	20.4	0.581883	105724	106692	1167-1173	1.000000	75.7
ccsA_p5	ccsA	5	CTCAAGCATCTCGGTTCGAA	GCGTTCAAGGGGTTCTCAAA	20	20	50.0	50.0	58.0	58.7	0.0	0.0	0.688978	105724	106692	1145-1151	1.000000	74.8
ndhH_p1	ndhH	1	AGGAGGGTCAAAATGGGTAGA	TTTGATGAGCCTCGCTTTGA	21	20	47.6	45.0	58.1	57.8	0.0	0.0	1.254177	113273	114454	1364-1364	1.000000	70.4
ndhH_p2	ndhH	2	AAAGGAGGGTCAAAATGGGT	TTTGATGAGCCTCGCTTTGA	20	20	45.0	45.0	56.9	57.8	0.0	0.0	1.277647	113273	114454	1366-1366	1.000000	70.2
ndhH_p3	ndhH	3	TCCCAAAAGGAGGGTCAAAA	TTTGATGAGCCTCGCTTTGA	20	20	45.0	45.0	56.8	57.8	0.0	0.0	1.340108	113273	114454	1371-1371	1.000000	69.7
ndhH_p4	ndhH	4	AGGAGGGTCAAAATGGGTAGA	TTGATGAGCCTCGCTTTGAG	21	20	47.6	50.0	58.1	58.3	0.0	0.0	1.340198	113273	114454	1363-1363	1.000000	69.7
ndhH_p5	ndhH	5	AAAGGAGGGTCAAAATGGGT	TTGATGAGCCTCGCTTTGAG	20	20	45.0	50.0	56.9	58.3	0.0	0.0	1.363668	113273	114454	1365-1365	1.000000	69.6