Hotspot

ndhF

SSC · 114616-116859 · 2244 bp

Back to Karpatiosorbus

Pi 0.000891
MarkerSeek score 57.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2244
variable_sites
2
pi
0.000891
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TCTGGATGGGAATCAAGAACA AACCGACCCCTTTCAAAAGA 42.9 45.0 56.6 57.3 2370-2382 bp 1.0 55.1
ndhF_p2 TCTGGATGGGAATCAAGAACA ACCGACCCCTTTCAAAAGAA 42.9 45.0 56.6 57.3 2369-2381 bp 1.0 55.1
ndhF_p3 TCTGGATGGGAATCAAGAACAA ACCGACCCCTTTCAAAAGAA 40.9 45.0 57.3 57.3 2369-2381 bp 1.0 52.9
ndhF_p4 TGGATGGGAATCAAGAACAAGA AACCGACCCCTTTCAAAAGA 40.9 45.0 57.3 57.3 2368-2380 bp 1.0 52.9
ndhF_p5 TGGATGGGAATCAAGAACAAGA ACCGACCCCTTTCAAAAGAA 40.9 45.0 57.3 57.3 2367-2379 bp 1.0 52.9