Hotspot

atpI

LSC · 15748-16491 · 744 bp

Back to Karpatiosorbus

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	CATTGCGTCAGGCTAAGACT	ACAATTCAAGTAGGCAAGTCA	50.0	38.1	58.0	55.4	855 bp	1.0	51.4
atpI_p2	TTACCTTGAGTCGCGCATAC	ACAATTCAAGTAGGCAAGTCA	50.0	38.1	58.1	55.4	874 bp	1.0	50.8
atpI_p3	CATTGCGTCAGGCTAAGACT	ACAATTCAAGTAGGCAAGTCAA	50.0	36.4	58.0	56.2	855 bp	1.0	49.5
atpI_p4	ACATTGCGTCAGGCTAAGAC	ACAATTCAAGTAGGCAAGTCA	50.0	38.1	58.3	55.4	856 bp	1.0	49.3
atpI_p5	TTACCTTGAGTCGCGCATAC	ACAATTCAAGTAGGCAAGTCAA	50.0	36.4	58.1	56.2	874 bp	1.0	49.0