Hotspot

rpl36-infA

LSC · 83620-83889 · 270 bp

Back to Karpatiosorbus

Pi 0.0037

MarkerSeek score 54.60

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 270
variable_sites: 1
pi: 0.0037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CGAACGGAGGCCCTTATTTT	TCAAAGCGGTTTTACCTGGA	50.0	45.0	58.3	57.4	397 bp	1.0	73.0
rpl36-infA_p2	ACTATAATTCGTCCCCGCCT	TCAAAGCGGTTTTACCTGGA	50.0	45.0	58.3	57.4	448 bp	1.0	72.7
rpl36-infA_p3	GCCTACGGATCAATCGACAT	TCAAAGCGGTTTTACCTGGA	50.0	45.0	57.6	57.4	432 bp	1.0	71.5
rpl36-infA_p4	CGAACGGAGGCCCTTATTTT	AGCGGTTTTACCTGGAATGA	50.0	45.0	58.3	57.1	393 bp	1.0	71.2
rpl36-infA_p5	TACGAACGGAGGCCCTTATT	TCAAAGCGGTTTTACCTGGA	50.0	45.0	58.5	57.4	399 bp	1.0	71.0