Hotspot

accD-psaI

LSC · 62243-63044 · 802 bp

Back to Karpatiosorbus

Pi 0

MarkerSeek score 61.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.4	58.0	908-934 bp	1.0	79.1
accD-psaI_p2	AGGGCGTTCTGAGTGAGTTA	AATGGAGGGGAGACTGTTGA	50.0	50.0	58.4	58.0	865-891 bp	1.0	79.0
accD-psaI_p3	AGGGCGTTCTGAGTGAGTTA	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.4	58.0	899-925 bp	1.0	78.9
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	GAGGGGAGACTGTTGAGAGT	50.0	55.0	58.4	58.1	861-887 bp	1.0	78.9
accD-psaI_p5	AGGGCGTTCTGAGTGAGTTA	GCACAAAAATGGAGGGGAGA	50.0	50.0	58.4	58.1	872-898 bp	1.0	78.8