Hotspot

ndhC-trnV-UAC

LSC · 54184-54906 · 723 bp

Back to Karpatiosorbus

Pi 0.00858

MarkerSeek score 66.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 6
pi: 0.00858
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	904-918 bp	1.0	80.2
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	840-854 bp	1.0	80.1
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	907-921 bp	1.0	79.0
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	906-920 bp	1.0	79.0
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	58.3	58.2	921-935 bp	1.0	78.6