Hotspot

ycf3-trns-GGA

LSC · 47348-47963 · 616 bp

Back to Karpatiosorbus

Pi 0

MarkerSeek score 59.10

Primer available yes

Label ycf3-trns-GGA

Feature Metadata

label_name: ycf3-trns-GGA
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trns-GGA_p1	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	807-809 bp	1.0	77.5
ycf3-trns-GGA_p2	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	806-808 bp	1.0	77.5
ycf3-trns-GGA_p3	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	757-759 bp	1.0	77.2
ycf3-trns-GGA_p4	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	756-758 bp	1.0	77.2
ycf3-trns-GGA_p5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.8	58.5	759-761 bp	1.0	74.4