Hotspot

petN-psbM

LSC · 30266-31521 · 1256 bp

Back to Karpatiosorbus

Pi 0.00478

MarkerSeek score 61.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1256
variable_sites: 6
pi: 0.00478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1362-1365 bp	1.0	76.6
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1305-1308 bp	1.0	75.9
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1304-1307 bp	1.0	75.9
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.8	57.6	1303-1306 bp	1.0	75.5
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	60.0	45.0	58.2	57.6	1300-1303 bp	1.0	75.2