Hotspot

rpl16

LSC · 81840-83297 · 1458 bp

Back to Kalidium

Pi 0.0174

MarkerSeek score 56.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 25
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCTCGGGCAATCG	AAACCATTCGCGCGAAAATT	50.0	40.0	58.1	57.9	2135-2153 bp	1.0	81.1
rpl16_p2	GGTGCCCCCACAATTTGATA	AAACCATTCGCGCGAAAATT	50.0	40.0	58.1	57.9	2328-2354 bp	1.0	80.7
rpl16_p3	TCTCAATTCTCGGGCAATCG	GCCGAATTTATAGCCGGACA	50.0	50.0	58.1	58.1	2313-2331 bp	1.0	80.5
rpl16_p4	TCTCAATTCTCGGGCAATCG	TGCCGAATTTATAGCCGGAC	50.0	50.0	58.1	58.1	2314-2332 bp	1.0	80.5
rpl16_p5	TCTCAATTCTCGGGCAATCG	ATTGCAGGTCGTATCGATGG	50.0	50.0	58.1	57.8	2205-2223 bp	1.0	80.3