Hotspot

ccsA-ndhD

SSC · 115791-116040 · 250 bp

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Pi 0.0245
MarkerSeek score 56.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
250
variable_sites
6
pi
0.0245
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGGGTTACATAGTTATGGTTCA CCCGGATTTCGTTCTCTCAC 36.4 55.0 54.2 58.4 349-354 bp 1.0 42.5
ccsA-ndhD_p2 AGGGTTACATAGTTATGGTTCA GGTGTTTACCCGGATTTCGT 36.4 50.0 54.2 58.2 357-362 bp 1.0 42.4
ccsA-ndhD_p3 AGGGTTACATAGTTATGGTTCA TGGTGTTTACCCGGATTTCG 36.4 50.0 54.2 58.2 358-363 bp 1.0 42.4
ccsA-ndhD_p4 AGGGTTACATAGTTATGGTTCAT GGTGTTTACCCGGATTTCGT 34.8 50.0 54.8 58.2 357-362 bp 1.0 42.4
ccsA-ndhD_p5 AGGGTTACATAGTTATGGTTCAT TGGTGTTTACCCGGATTTCG 34.8 50.0 54.8 58.2 358-363 bp 1.0 42.4