Hotspot

rpl32-trnL-UAG

SSC · 113783-114595 · 813 bp

Back to Kalidium

Pi 0.00658
MarkerSeek score 64.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
813
variable_sites
5
pi
0.00658
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCCTTTT CTCGCACTGCTTCCTAAGAG 45.0 55.0 57.9 58.1 899-948 bp 1.0 82.6
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCCTTTT CTCTCGCACTGCTTCCTAAG 45.0 55.0 57.9 58.1 901-950 bp 1.0 82.6
rpl32-trnL-UAG_p3 GGCAGCGTTAAAAGCCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 888-937 bp 1.0 82.3
rpl32-trnL-UAG_p4 GGCAGCGTTAAAAGCCTTTT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.9 57.8 881-930 bp 1.0 81.8
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.9 57.8 886-935 bp 1.0 81.7