Hotspot

ndhF-rpl32

SSC · 112476-113620 · 1145 bp

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Pi 0.021

MarkerSeek score 71.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1145
variable_sites: 24
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAACTTGGAGTGGAAGGA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.9	57.9	1299-1313 bp	1.0	79.6
ndhF-rpl32_p2	CGCCGAAGATTCTTTGTTGC	AAAAGGCTTTTAACGCTGCC	50.0	45.0	58.4	57.9	1355-1369 bp	1.0	77.3
ndhF-rpl32_p3	AGGAACTTGGAGTGGAAGGA	TTTAACGCTGCCCAATACCC	50.0	50.0	57.9	58.5	1291-1305 bp	1.0	76.5
ndhF-rpl32_p4	CGCCGAAGATTCTTTGTTGC	TTTAACGCTGCCCAATACCC	50.0	50.0	58.4	58.5	1347-1361 bp	1.0	74.2
ndhF-rpl32_p5	AGGAACTTGGAGTGGAAGGA	GCTTTTAACGCTGCCCAATA	50.0	45.0	57.9	57.1	1294-1308 bp	1.0	73.3