Hotspot

trnN-GUU-ndhF

IRb · 108384-110234 · 1851 bp

Back to Kalidium

Pi 0.0103

MarkerSeek score 56.20

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1851
variable_sites: 19
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GAGGGGGTCGAATTTCTTCC	50.0	55.0	58.2	58.0	2000-2005 bp	1.0	79.4
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	GGAGGGGGTCGAATTTCTTC	50.0	55.0	58.2	58.0	2001-2006 bp	1.0	79.4
trnN-GUU-ndhF_p3	AATCAGTTAACAGCCGACCG	GAGGGGGTCGAATTTCTTCC	50.0	55.0	58.3	58.0	1981-1986 bp	1.0	78.4
trnN-GUU-ndhF_p4	AATCAGTTAACAGCCGACCG	GGAGGGGGTCGAATTTCTTC	50.0	55.0	58.3	58.0	1982-1987 bp	1.0	78.4
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	GAGGGGGTCGAATTTCTTCC	55.0	55.0	57.7	58.0	2008-2013 bp	1.0	78.0