Hotspot

trnM-CAU-atpE

LSC · 52559-52848 · 290 bp

Back to Kalidium

Pi 0.0186

MarkerSeek score 64.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 290
variable_sites: 5
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GGCGAGAGTCATTGGTTCAA	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.2	58.0	360-375 bp	1.0	83.3
trnM-CAU-atpE_p2	GGCGAGAGTCATTGGTTCAA	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.2	58.1	352-367 bp	1.0	83.1
trnM-CAU-atpE_p3	GGCGAGAGTCATTGGTTCAA	AGCGACAAGGAATTGAAGCA	50.0	45.0	58.2	58.1	387-402 bp	1.0	82.7
trnM-CAU-atpE_p4	GGCGAGAGTCATTGGTTCAA	AAGCTAACTTGAGGAAGGCG	50.0	50.0	58.2	57.9	414-429 bp	1.0	82.6
trnM-CAU-atpE_p5	GGCGAGAGTCATTGGTTCAA	GGAAGGAAAGCGACAAGGAA	50.0	50.0	58.2	58.1	395-410 bp	1.0	82.6