Hotspot

trnS-GCU-trnG-GCC

LSC · 8163-9100 · 938 bp

Back to Kalidium

Pi 0.0107

MarkerSeek score 54.30

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 10
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	1061-1064 bp	1.0	51.6
trnS-GCU-trnG-GCC_p2	TGGAAACGGAAAGAGAGGGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.3	55.5	1066-1069 bp	1.0	50.6
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	AGAACGAATCACACTTTTACCAC	50.0	39.1	58.2	57.3	1064-1067 bp	1.0	50.6
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.2	57.3	1063-1066 bp	1.0	50.6
trnS-GCU-trnG-GCC_p5	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	1062-1065 bp	1.0	49.7