Hotspot

ndhG-ndhI

SSC · 116004-116236 · 233 bp

Back to Kalanchoe

Pi 0.0576

MarkerSeek score 67.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 233
variable_sites: 33
pi: 0.0576
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCATCATTGACCGACTCCTT	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1382-1429 bp	1.0	87.6
ndhG-ndhI_p2	GCATCATTGACCGACTCCTT	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1381-1428 bp	1.0	87.6
ndhG-ndhI_p3	GCATCATTGACCGACTCCTT	GCTGCAAGATACATCGGTCA	50.0	50.0	58.0	58.1	1368-1415 bp	1.0	87.6
ndhG-ndhI_p4	CGAAAGCTTTGTTACTGCCG	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1206-1248 bp	0.714286	75.0
ndhG-ndhI_p5	CGAAAGCTTTGTTACTGCCG	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1205-1247 bp	0.714286	75.0