Hotspot

rpl32-trnL-UAG

SSC · 110992-111446 · 455 bp

Back to Kalanchoe

Pi 0.0343

MarkerSeek score 74.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 455
variable_sites: 51
pi: 0.0343
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCCAAATCCCTTTCTACTGGT	TTCGAACCGAGATGCTCAAG	47.6	50.0	57.9	58.0	578-619 bp	1.0	82.0
rpl32-trnL-UAG_p2	GCCAAATCCCTTTCTACTGGT	AAGCACTGCTTCCTAAGAGC	47.6	50.0	57.9	57.9	561-602 bp	1.0	81.3
rpl32-trnL-UAG_p3	GCCAAATCCCTTTCTACTGGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	551-592 bp	1.0	81.3
rpl32-trnL-UAG_p4	GCCAAATCCCTTTCTACTGGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	544-585 bp	1.0	80.9
rpl32-trnL-UAG_p5	GCCAAATCCCTTTCTACTGGT	GCTCAAGCACTGCTTCCTAA	47.6	50.0	57.9	58.2	565-606 bp	1.0	80.7